Coding

Part:BBa_K4623001:Design

Designed by: Yixin Huang   Group: iGEM23_BNU-China   (2023-10-08)


Monomeric streptavidin(mSA)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 82
    Illegal AgeI site found at 142
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We have performed codon optimization on the obtained amino acid sequence.


Source

We got the sequence from https://pubmed.ncbi.nlm.nih.gov/22806584/

References

[1]Lim KH, Huang H, Pralle A, Park S. Stable, high-affinity streptavidin monomer for protein labeling and monovalent biotin detection. Biotechnol Bioeng. 2013 Jan;110(1):57-67. doi: 10.1002/bit.24605. Epub 2012 Aug 8. PMID: 22806584.